JNTUH B.Tech 2nd Year 1 sem Biotechnology Engineering (2-1) Microbiology Engineering R13.

JNTUH B.Tech 2nd year Microbiology gives you detail information about Microbiology Engineering R13 year subject. It will be help full you to understand you complete curriculum of the year.

Objectives

This course is formulated to provides an exposure to microbial diversity, their characteristics, classification, physiology and identification. It also enables them to understand host pathogen interaction and their microbial environment. It also introduces the students to pathogens and viruses.

UNIT I: INTRODUCTION TO MICROBIOLOGY

Discovery of micro organisms; Theory of spontaneous generation, Germ theory of diseases; Major contribution and events in the field of Microbiology. Scope and relevance of microbiology. Identification of Micro organisms – A general account. Micro diversity

UNIT II: MAJOR GROUPS OF MICRO ORGANISMS AND GENERAL METHODS OF IDENTIFICATION

General characteristics of Bacteria, Archaea and Eubacteria. Diversity classification of Woese et al. Three domains of life. Five – kingdom system of Whittaker. Classification systems – Phylogenetic, Phenetic, Taxonomic ranks, Major characteristics used in Taxonomy – Morphological, Physiological, ecological, Biochemical, Immunological, Genetic and Molecular. Colony characteristics, staining techniques; Fixation, Principle dyes, simple staining, differential staining, spore staining, flageller staining. Biochemical tests – Sugar fermentations, IMVIC tests, Catalase production etc.

UNIT III: NUTRITION, CULTIVATION AND PRESERVATION OF MICROORGANISMS

Nutrition requirements of micro organisms; nutritional classes of microbes, Macro and micro nutrients, their sources and physiological functions of nutrients. Growth factors and their functions in metabolism. Aerobic and anaerobic metabolism.
Cultivation of micro organisms; Culture media, synthetic, complex media, solidifying agents, types of media – selective, differential and enrichment and enriched media, pure culture methods – spread plate, pour plate and streak plate, special techniques for cultivation of anaerobes. Influence of environmental factors on growth – solutes, water activity, pH, temperature, oxyzen, osomotic pressure, radiation. Preservation of Microganisms: working and primary stock cultures – agar slants, agar stabs, spore preparation, use of sterile soil, cryopreservation, lyophilisation, Application and limitations of various methods.

Download iStudy Android App for complete JNTUH syllabus, results, timetables and all other updates. There are no ads and no pdfs and will make your life way easier.

TEXT BOOKS

1. Microbiology, Pelczar M.J. Chan ECE and Krieg NR. Tata McGraw Hill.
2. General Microbiology. Roger Y stanier, Macmillan.

REFERENCES

1.  Biology of Micro organisma. BROCK, Prentice Hall, International Inc.
2.  Introduction to Micro Biology a case History approach 3rd edition john. L. Ingraham, Catherine A lingraham, Thomson Publications.
3. General Microbiology. Hons. G.Schlege. Combridge university press.
4. General Microbiology. Prescott and Dunn Mc Graw Hill Publishers.
5. Introduction to microbiology – A case history approach 3rd edition – john L. ingram, Catherine A. Ingram Thomson Publishers.
6. Microbiology: A Human perspective 5th edition, Nestor, Anderson, Robertes

Outcomes

Student would be able to distinguish between bacteria by culture and staining methods. They understand the different mechanisms associated with pathogenecity, virus replication, quantification and identification. They will gain the ability to differentiate virus from bacteria and also correlate the differences between replication, quantification and identification methods.

For more information about all JNTU updates please stay connected to us on FB and don’t hesitate to ask any questions in the comment. 🙂