JNTUH B.Tech 2nd year Applied Bio chemistry Engineering R13 gives you detail information about Applied Bio chemistry Engineering R13 year subject. It will be help full you to understand you complete curriculum of the year.
UNIT-I :Properties of water
pH & Buffers, Physiological buffer. The Handerson Hasselbalch equation, determination of
pKa values. Structural aspects of carbohydrates , amino acids and lipids. Carbohydrate metabolism: respirationtypes.
glycolysis and kreb’s cycle and energetics involved, Protein & Lipid metabolism (Briefly).
UNIT-II: Biochemistry of Living Cell
types of cells, pro and eu karyotes. Sub-cellular – Fractionation using the Differential
Centrifugation Method. Functions of each Organelle, Chemical Composition of cell walls Membrane lipids.
Transport of Substances across Biological Membrane. Redox potential, components in electron transport
systems in mitochondria, respiratory chain. Oxidative phosphorylation – Energetics ,
UNIT-III : Chemical nature of Enzymes
study of the Properties of Enzymes and kinetics by Spectrophotometer. Diagnostic and therapeutic uses of Enzymes. Metalions in biological catalysis ( explanation with few examples )
TEXT BOOKS:
- Lehninger A.L, Nelson O.’L. M.M. Cox, Principles of Biochemistry 3rd edition, 2000 CBS
- Robert Murray, Peter A. Mayes, Victor W. Rodwell, Daryl K. Granner, Harper’s Biochemistry, 26th Edition,
McGraw-Hill Companies, February 2003.
REFERENCES:
- Rao, N. Mallikarjuna, Medical Biochemistry, New Age International Pvt Ltd,2002.
- Lalit M. Srivastava, Nibhriti Das & Subrata sinha, Essentials of practical Biochemistry, CBS Publishers, Ist
edition, 2002. - Bertini I, Gray HB, Stiefel EI, Valentine JS Biological inorganic chemistry. University Science Books,
Sausalito, 2006. - Frausto da Silva JJR, Williams RJP The biological chemistry of the elements: the inorganic chemistry of life,
Oxford University Press, New York, 2001 - Bertini I, Sigel A, Sigel H Handbook on metalloproteins. Marcel Dekker, New York ,2001
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