Pharmaceutical Microbiology Lab Detailed Syllabus for B.Tech third year first sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Pharmaceutical Microbiology Lab B.Tech 2016-2017 (R16) third year first sem is as follows.
B.Tech. III Year I Sem. L/T/P/C
Course Code:PS508 0/0/3/2
- Introduction to equipment and glassware used in microbiology laboratory.
- Study of morphology of different microbes
- Preparation of various culture media, cultivation of microbes and observation of colony characteristics.
- Sterilization techniques (moist and dry heat) and their validations.
- Aseptic transfer of culture into different types of media.
- Characterisation of microbes by staining techniques (simple, gram’s, acid fast and negative staining).
- Study of motility of bacteria by hanging drop method.
- Characterization of microbes through Bio chemical reactions:
a. Indole test.
b. Methyl red test.
c. Voges proskauer test.
d. Starch hydrolysis test.
e. Fermentation of carbohydrates. - Isolation of pure cultures by streak plate, spread plate & pour plate techniques.
- Enumeration of bacteria by pour plate/spread plate technique
- Enumeration of bacteria by direct microscopic count.
- Evaluation of any disinfectant by phenol coefficient test
- Study of Oligodynamic action (of metals on bacteria)
- Preservation of microrganisms (slant and stab cultures)
- Microbiological Analysis of Water.
REFERENCE
- Garg,F C Experimental Microbiology
- Gaud.R.S, Gupta G.D, Practical Microbiology
- Vanitha Kale and kishore Bhusari, Pratical microbiology principles and Techniques
For all other B.Tech 3rd Year 1st Sem syllabus go to JNTUH B.Tech B. Pharm 3rd Year 1st Sem Course Structure for (R16) Batch.
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