{"id":61359,"date":"2023-09-06T17:17:04","date_gmt":"2023-09-06T17:17:04","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/bt3011-modern-bio-analytical-techniques-syllabus-for-biotech-2021-regulation-professional-elective-ii\/"},"modified":"2023-09-06T17:17:04","modified_gmt":"2023-09-06T17:17:04","slug":"bt3011-modern-bio-analytical-techniques-syllabus-for-biotech-2021-regulation-professional-elective-ii","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/bt3011-modern-bio-analytical-techniques-syllabus-for-biotech-2021-regulation-professional-elective-ii\/","title":{"rendered":"BT3011: Modern Bio Analytical Techniques syllabus for BioTech 2021 regulation (Professional Elective-II)"},"content":{"rendered":"

Modern Bio Analytical Techniques detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities<\/a> official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below. <\/p>\n

For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme<\/a>. For Professional Elective-II scheme and its subjects refer to BioTech Professional Elective-II syllabus scheme<\/a>. The detailed syllabus of modern bio analytical techniques is as follows. <\/p>\n

Course Objectives:<\/h4>\n

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Unit I<\/h4>\n

SPECTROSCOPY STUDY OF CHEMICAL COMPOUNDS AND BIO-MOLECULES\t9<\/strong>
\nElectromagnetic radiations and interactions with matters: Electromagnetic spectrum. Quantisation of energy, Electronic, vibrational and rotational spectroscopy. Franck-Condon principle, Jablonski diagram, radiative, nonradiative pathways, fluorescence and phosphorescence. Absorption of radiation, BeerLambert\u2019s law, deviation of Beer-Lambert\u2019s equation and its limitations. Principals, instrumentation, sampling and application of few spectroscopic techniques: UV-Visible spectroscopy, Fluorescence spectroscopy, IR\/Raman spectroscopy, NMR Spectroscopy and Mass spectroscopy.\n<\/p>\n

Unit II<\/h4>\n

DIFFRACTION TECHNIQUE\t9<\/strong>
\nIntroduction to lattice and lattice systems, Bragg\u2019s plane, miller indices, point groups and space groups Principle of diffraction and X-ray diffraction: X-rays production, X- ray spectra, Bragg\u2019s law and intensity of X- rays, Mosley\u2019s law, powdered XRD, percentage crystallinity, single crystal XRD, macromolecular XRD (protein crystallization, data collection and structure solution).\n<\/p>\n

Unit III<\/h4>\n

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Unit IV<\/h4>\n

MICROSCOPY\t9<\/strong>
\nMicroscopy with light and electrons – Electrons and their interaction with the specimen – Electron, diffraction – Instrument, specimen preparation and application of TEM and SEM – Fluorescence microscopy – Laser confocal microscopy – Phase contrast – Video microscopy – Scanning probe microscopy.\n<\/p>\n

Unit V<\/h4>\n

ELECTROPHORETIC TECHNIQUES\t9<\/strong>
\nPrinciple, equipment and process, Agarose gel electrophoresis, horizontal and vertical gel electrophoresis, electrophoresis techniques, Isoelectric focusing, capillary electrophoresis and application of electrophoresis in analysing macromolecules.\n<\/p>\n

Course Outcomes:<\/h4>\n