{"id":60584,"date":"2023-09-06T12:07:15","date_gmt":"2023-09-06T12:07:15","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/bt3761-downstream-laboratory-syllabus-for-biotech-2021-regulation\/"},"modified":"2023-09-06T12:07:15","modified_gmt":"2023-09-06T12:07:15","slug":"bt3761-downstream-laboratory-syllabus-for-biotech-2021-regulation","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/bt3761-downstream-laboratory-syllabus-for-biotech-2021-regulation\/","title":{"rendered":"BT3761: Downstream Laboratory syllabus for BioTech 2021 regulation"},"content":{"rendered":"<p align=\"justify\">Downstream Laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Biotechnology 7th Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/biotech-7th-sem-syllabus-2021-regulation\">BioTech 7th Sem 2021 regulation scheme<\/a>. The detailed syllabus of downstream laboratory is as follows. <\/p>\n<p><h4>Course Objectives:<\/h4>\n<ul>\n<li>To provide hands on training in Down stream processing through simple experimentations inthe laboratory. This will be a pre-requisite for project work.<\/li>\n<li>The objectives of this course is to practice the students<\/li>\n<li>To understand the nature of the end product, its concentration, stability and degree of purification required<\/li>\n<li>To design processes for the recovery and subsequent purification of target biological products.<\/li>\n<\/ul>\n<p><h4>Experiments<\/h4>\n<ol>\n<li>Solid liquid separation &#8211; centrifugation<\/li>\n<li>Solid liquid separation &#8211; microfiltration<\/li>\n<li>Cell disruption techniques &#8211; ultrasonication or French pressure cell or Dynomill<\/li>\n<li>Precipitation &#8211; ammonium sulphite precipitation<\/li>\n<li>Ultra filtration separation<\/li>\n<li>Aqueous two phase extraction of biologicals<\/li>\n<li>High resolution purification &#8211; affinity chromatography<\/li>\n<li>High resolution purification &#8211; ion exchange chromatography<\/li>\n<li>Product polishing &#8211; spray drying or freeze drying<\/li>\n<li>Size exclusion chromatography<\/li>\n<\/ol>\n<p><h4>Course Outcomes:<\/h4>\n<p>Upon success completion of this course, the students would have<\/p>\n<ol>\n<li>Acquired knowledge for the separation of whole cells and other insoluble ingredients from the culture broth.<\/li>\n<li>Learned cell disruption techniques to release intracellular products<\/li>\n<li>Learned various techniques like evaporation, extraction, precipitation, membrane separation for concentrating biological products<\/li>\n<li>Learned the basic principles and techniques of chromatography to purify the biological products and formulate the products for different end uses.<\/li>\n<\/ol>\n<p><h4>Reference Books:<\/h4>\n<ol>\n<li>P.A. Belter, E.L. Cussler And Wei-Houhu &#8211; Bioseparations &#8211; Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).<\/li>\n<li>J.C. Janson And L. Ryden, (Ed.) &#8211; Protein Purification &#8211; Principles, High ResolutionMethods And Applications, VCH Pub. 1989.<\/li>\n<li>Michael R.Ladisch Bioseparations Engineering: Principles, practice and Economics, Wiley-Interscience,1st Edition,2001.<\/li>\n<li>Product Recovery in Bioprocess Technology\u201d. (BIOTOL &#8211; Biotechnology by Open Learning Series). Butterworth &#8211; Heinmann \/ Elsevier, 2004.<\/li>\n<li>Raja Ghosh \u201cPrinciples of Bioseparations Engineering\u201d. World Scientific, 2006<\/li>\n<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Biotechnology, 2021 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/biotech+7th-sem\">BioTech 7th Sem subject syllabuses for 2021 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Biotechnology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University BioTech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Downstream Laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BioTech students. For course code, course [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[50,81],"tags":[],"class_list":["post-60584","post","type-post","status-publish","format-standard","hentry","category-7th-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/60584","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=60584"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/60584\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=60584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=60584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=60584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}