{"id":60572,"date":"2023-09-06T12:06:44","date_gmt":"2023-09-06T12:06:44","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/bt3511-molecular-biology-and-genetic-engineering-laboratory-syllabus-for-biotech-2021-regulation\/"},"modified":"2023-09-06T12:06:44","modified_gmt":"2023-09-06T12:06:44","slug":"bt3511-molecular-biology-and-genetic-engineering-laboratory-syllabus-for-biotech-2021-regulation","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/bt3511-molecular-biology-and-genetic-engineering-laboratory-syllabus-for-biotech-2021-regulation\/","title":{"rendered":"BT3511: Molecular Biology and Genetic Engineering laboratory syllabus for BioTech 2021 regulation"},"content":{"rendered":"<p align=\"justify\">Molecular Biology and Genetic Engineering laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Biotechnology 5th Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/biotech-5th-sem-syllabus-2021-regulation\">BioTech 5th Sem 2021 regulation scheme<\/a>. The detailed syllabus of molecular biology and genetic engineering laboratory is as follows. <\/p>\n<p><h4>Course Objectives:<\/h4>\n<h4 id=\"istudy\" style=\"text-align:center\"><a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Download the iStudy App for all syllabus and other updates.<\/a><br \/><a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy&amp;pcampaignid=pcampaignidMKT-Other-global-all-co-prtnr-py-PartBadge-Mar2515-1\/\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/play.google.com\/intl\/en_us\/badges\/static\/images\/badges\/en_badge_web_generic.png\" alt=\"Get it on Google Play\" style=\"height:65px;text-align:center\"><\/a><\/h4>\n<p><h4>List of Experiments:<\/h4>\n<ol>\n<li>Isolation of total DNA<\/li>\n<li>Isolation of Plasmid DNA<\/li>\n<li>Agarose gel electrophoresis<\/li>\n<li>Restriction enzyme digestion of DNA<\/li>\n<li>DNA ligation<\/li>\n<li>Competent cell preparation &amp; Transformation<\/li>\n<li>Blue-White screening<\/li>\n<li>Induction and Analysis of Gene expression<\/li>\n<li>PCR<\/li>\n<li>SDS-PAGE<\/li>\n<li>Western blot<\/li>\n<\/ol>\n<p><h4>Course Outcomes:<\/h4>\n<p>Upon completion of the course, students will be able to<\/p>\n<ul>\n<li>Understand basic techniques of DNA isolation and manipulation.<\/li>\n<li>Gain experience in selecting genetically transformed organisms for downstream analysis.<\/li>\n<li>Aware basic techniques involved in analysis of gene expression at nucleic acids and proteins level<\/li>\n<li>Establish the ability to carry out laboratory experiments and interpret the results.<\/li>\n<li>Apply practical knowledge to solve biotechnological problems.<\/li>\n<li>Aware of the hazardous chemicals and safety precautions in case of emergency.<\/li>\n<\/ul>\n<p><h4>Text Books:<\/h4>\n<h4 id=\"istudy\" style=\"text-align:center\"><a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Download the iStudy App for all syllabus and other updates.<\/a><br \/><a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy&amp;pcampaignid=pcampaignidMKT-Other-global-all-co-prtnr-py-PartBadge-Mar2515-1\/\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/play.google.com\/intl\/en_us\/badges\/static\/images\/badges\/en_badge_web_generic.png\" alt=\"Get it on Google Play\" style=\"height:65px;text-align:center\"><\/a><\/h4>\n<p><h4>Reference Books:<\/h4>\n<ol>\n<li>Keith Wilson and John Walker, \u201cPrinciples and Techniques of Biochemistry and Molecular Biology\u201d 8th Edition, Cambridge University Press, 2018.<\/li>\n<li>Alan S. Gerstein, \u201cMolecular Biology Problem Solver-A Laboratory Guide\u201d Wiley-Liss, Inc., 2017.<\/li>\n<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Biotechnology, 2021 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/biotech+5th-sem\">BioTech 5th Sem subject syllabuses for 2021 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Biotechnology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University BioTech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Molecular Biology and Genetic Engineering laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BioTech students. [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[48,81],"tags":[],"class_list":["post-60572","post","type-post","status-publish","format-standard","hentry","category-5th-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/60572","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=60572"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/60572\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=60572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=60572"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=60572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}