{"id":51788,"date":"2023-03-22T07:43:15","date_gmt":"2023-03-22T07:43:15","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/bt3362-cell-and-microbiology-laboratory-syllabus-for-biotech-2021-regulation\/"},"modified":"2023-03-22T07:43:15","modified_gmt":"2023-03-22T07:43:15","slug":"bt3362-cell-and-microbiology-laboratory-syllabus-for-biotech-2021-regulation","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/bt3362-cell-and-microbiology-laboratory-syllabus-for-biotech-2021-regulation\/","title":{"rendered":"BT3362: Cell and Microbiology Laboratory syllabus for BioTech 2021 regulation"},"content":{"rendered":"<p align=\"justify\">Cell and Microbiology Laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Biotechnology 3rd Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/biotech-3rd-sem-syllabus-2021-regulation\">BioTech 3rd Sem 2021 regulation scheme<\/a>. The detailed syllabus of cell and microbiology laboratory is as follows. <\/p>\n<p>  <title>Cell and Microbiology Laboratory<\/title><\/p>\n<h4>Course Objectives:<\/h4>\n<ul>\n<li>To demonstrate various techniques to learn the morphology, identification and propagation of cells and microbes.<\/li>\n<li>To learn the staining techniques and culturing of microorganism.<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<ol>\n<li>Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques<\/li>\n<li>Microscopy &#8211; Working and care of Microscope, phase contrast and fluorescent microscopy<\/li>\n<li>Culture Media-Types and Use; Preparation of Nutrient broth and agar<\/li>\n<li>Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test tubes; Solid:Pour plates, streak plates, slants, stabs<\/li>\n<li>Identification of given plant, animal, bacterial cells and yeast\/mould<\/li>\n<li>Staining Techniques Simple, Differential- Grams Staining, spore \/capsule staining, Giemsa, and Leishman Staining<\/li>\n<li>Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil &#8211; TVC<\/li>\n<li>Effect of Disinfectants- Phenol Coefficient, Antibiotic Sensitivity Assay<\/li>\n<li>Osmosis and Tonicity and Tryphan Blue Assay<\/li>\n<li>Growth Curve in Bacteria and Yeast<\/li>\n<li>Staining for different stages of mitosis in AlliumCepa (Onion)<\/li>\n<li>Effect of pH, Temperature, UV radiation on Growth Bacteria<\/li>\n<\/ol>\n<h4>Equipment Needed for 30 Students<\/h4>\n<ol>\n<li>Autoclave 1<\/li>\n<li>Hot Air Oven 1<\/li>\n<li>Incubators 2<\/li>\n<li>Light Microscopes 4<\/li>\n<li>Incubator Shaker 1<\/li>\n<li>Colorimeter 2<\/li>\n<li>Lamina Flow Chamber 2<\/li>\n<li>Glassware: Petridish, Test tubes,Microscopic slides, Inoculation , loop, Gas burner<\/li>\n<li>Chemicals and media<\/li>\n<li>Bacterial culture media, Yeast culture media, 70% ethanol ,antibiotics, Crystal violet, Iodine, Safranin, India ink (capsule staining), Immersion oil<\/li>\n<\/ol>\n<h4>Course Outcomes:<\/h4>\n<p>  Students will be able to<\/p>\n<ul>\n<li>Understand the advanced technical information pertaining to laboratory bio-safety and preventive measures from pathogenic microorganism.<\/li>\n<li>Know the various aseptic techniques and sterilization methods.<\/li>\n<li>Develop the minimum skills to work on several important techniques for the study of microorganisms in the laboratory.<\/li>\n<li>Learn the various techniques of culturing of microorganisms and media preparation.<\/li>\n<li>Study the growth of microorganisms by varying the growth conditions.<\/li>\n<li>Identify the various stages of mitosis<\/li>\n<\/ul>\n<h4>Reference Books:<\/h4>\n<ol>\n<li>Cappuccino, J.G. and N. Sherman Microbiology: A Laboratory Manual, 4th Edition, Addison-Wesley, 1999.<\/li>\n<li>Collee, J.G. etal., Mackie &amp; McCartney Practical Medical Microbiology 4th Edition, ChurchillLivingstone, 1996Rickwood, D. and J.R. Harris Cell Biology: Essential Techniques, Johnwiley, 1996.<\/li>\n<li>Davis, J.M. Basic Cell Culture: A Practical Approach, IRL, 1994.<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Biotechnology, 2021 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/biotech+3rd-sem\">BioTech 3rd Sem subject syllabuses for 2021 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Biotechnology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University BioTech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cell and Microbiology Laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BioTech students. For course [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[46,81],"tags":[],"class_list":["post-51788","post","type-post","status-publish","format-standard","hentry","category-3rd-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/51788","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=51788"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/51788\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=51788"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=51788"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=51788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}