{"id":36761,"date":"2021-05-25T11:49:28","date_gmt":"2021-05-25T11:49:28","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/pm5412-cell-and-molecular-biology-laboratory-syllabus-for-pharma-tech-4th-sem-2019-regulation-anna-university\/"},"modified":"2021-05-25T11:49:28","modified_gmt":"2021-05-25T11:49:28","slug":"pm5412-cell-and-molecular-biology-laboratory-syllabus-for-pharma-tech-4th-sem-2019-regulation-anna-university","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/pm5412-cell-and-molecular-biology-laboratory-syllabus-for-pharma-tech-4th-sem-2019-regulation-anna-university\/","title":{"rendered":"PM5412: Cell and Molecular Biology Laboratory Syllabus for Pharma Tech 4th Sem 2019 Regulation Anna University"},"content":{"rendered":"<p align=\"justify\">Cell and Molecular Biology Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna Universities<\/a> official website and presented for the Pharma Tech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Pharmaceutical Technology 4th Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/pharmaceutical-technology-pharma-tech-syllabus-for-4th-sem-2019-regulation-anna-university\">Pharma Tech 4th Sem 2019 regulation scheme<\/a>. The detailed syllabus of cell and molecular biology laboratory is as follows. <\/p>\n<p>  <title>Cell and Molecular Biology Laboratory<\/title><\/p>\n<h4>Course Objective:<\/h4>\n<p align=\"justify\">\n  The course aims to,<\/p>\n<ul>\n<li>understand the principle of nucleic acid isolation.<\/li>\n<li>understd the principles of PCR and their uses in genetic engineering.<\/li>\n<li>gain a thorough knowledge about nucleic acid hybridization.<\/li>\n<li>learn the history of DNA sequencing and current methods and gene synthesis<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Preparation of Genomic DNA<\/li>\n<li>PCR amplification of gene from the genomic DNA<\/li>\n<li>Preparation of plasmid DNA<\/li>\n<li>Detection of Plasmid DNA by Agarose gel electrophoresis<\/li>\n<li>Restriction Digestion of the vector and Insert<\/li>\n<li>Ligation and Transformation ofE.coli<\/li>\n<li>Lysate PCR confirmation.<\/li>\n<li>Restriction and gel elution of DNA fragments<\/li>\n<li>Electroporation of Yeast<\/li>\n<li>SDS-PAGE analysis of purified protein<\/li>\n<li>Western blot confirmation of expressed protein (anti his)<\/li>\n<li>Quantification of proteins.<\/li>\n<li>RNA Isolation<\/li>\n<li>cDNA preparation from RNA<\/li>\n<li>Site directed mutagenesis<\/li>\n<li>Southern hybridization experiment<\/li>\n<\/ol>\n<h4>List of Equipments<\/h4>\n<p align=\"justify\">\n  REQUIRED FOR 30 STUDENTS<\/p>\n<ul>\n<li>PCR machine<\/li>\n<li>Electrophoretic assemblies for DNA and protein separations<\/li>\n<li>ELISA reader<\/li>\n<li>Ultracentrifuge<\/li>\n<li>Laminar air flow cabinets<\/li>\n<li>Cooling centrifuge<\/li>\n<\/ul>\n<h4>Course Outcome:<\/h4>\n<p align=\"justify\">\n  At the end of the course the students will be able to<\/p>\n<ol>\n<li>experience basic techniques of DNA isolation and manipulation<\/li>\n<li>experience in selecting genetically transformed organisms for downstream analysis<\/li>\n<li>experience basic techniques involved in analysis of gene expression at nucleic acids and proteins level<\/li>\n<\/ol>\n<h4>References:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Old RW, Primrose SB, &#8220;Principles of Gene Manipulation, &#8220;An Introduction to Genetic Engineering&#8221;, Blackwell Science Publications, 1993.<\/li>\n<li>Ausubel FM, Brent R, Kingston RE, Moore DD, &#8220;Current Protocols in Molecular Biology, &#8220;Greene Publishing Associates, NY, 2003.<\/li>\n<li>S. John Venison, &#8220;Laboratory manual for Genetic Engineering&#8221;, Eastern economy edition, 2009.<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Pharmaceutical Technology, 2019 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/pharma-tech+4th-sem\">Pharma Tech 4th Sem subject syllabuses for 2019 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Pharmaceutical Technology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University Pharma Tech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cell and Molecular Biology Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Pharma [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[47,142],"tags":[],"class_list":["post-36761","post","type-post","status-publish","format-standard","hentry","category-4th-sem","category-pharmaceutical-technology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/36761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=36761"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/36761\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=36761"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=36761"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=36761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}