{"id":36746,"date":"2021-05-25T11:49:18","date_gmt":"2021-05-25T11:49:18","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/ib5361-biochemistry-laboratory-syllabus-for-pharma-tech-2nd-sem-2019-regulation-anna-university\/"},"modified":"2021-05-25T11:49:18","modified_gmt":"2021-05-25T11:49:18","slug":"ib5361-biochemistry-laboratory-syllabus-for-pharma-tech-2nd-sem-2019-regulation-anna-university","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/ib5361-biochemistry-laboratory-syllabus-for-pharma-tech-2nd-sem-2019-regulation-anna-university\/","title":{"rendered":"IB5361: Biochemistry Laboratory Syllabus for Pharma Tech 2nd Sem 2019 Regulation Anna University"},"content":{"rendered":"<p align=\"justify\">Biochemistry Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna Universities<\/a> official website and presented for the Pharma Tech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Pharmaceutical Technology 2nd Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/pharmaceutical-technology-pharma-tech-syllabus-for-2nd-sem-2019-regulation-anna-university\">Pharma Tech 2nd Sem 2019 regulation scheme<\/a>. The detailed syllabus of biochemistry laboratory is as follows. <\/p>\n<p>  <title>Biochemistry Laboratory<\/title><\/p>\n<h4>Course Objective:<\/h4>\n<p align=\"justify\">\n  The course aims to,<\/p>\n<ul>\n<li>learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,).<\/li>\n<li>make students to learn the basic units of measurements and standardisation of various buffer solutions<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>General guidelines for working in biochemistry lab (theory)<\/li>\n<li>Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.<\/li>\n<li>Accuracy, precision, sensitivity and specificity (theory)<\/li>\n<li>Preparation of buffer -titration of a weak acid and a weak base.<\/li>\n<li>Qualitative tests for carbohydrates &#8211; distinguishing reducing from non-reducing sugars and keto from aldo sugars.<\/li>\n<li>Quantitative method for amino acid estimation using ninhydrin &#8211; distinguishing amino from imino acid.<\/li>\n<li>Protein estimation by Biuret and Lowry&#8221;s methods.<\/li>\n<li>Protein estimation by Bradford and spectroscopic methods.<\/li>\n<li>Extraction of lipids and analysis by TLC.<\/li>\n<li>Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).<\/li>\n<li>Enzymatic assay: phosphatase from potato.<\/li>\n<li>Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.<\/li>\n<\/ol>\n<h4>Equipment Needed for 20 Students<\/h4>\n<p align=\"justify\">\n  Autoclave 1<br \/>\n  Hot Air Oven 1<br \/>\n  Incubators 2<br \/>\n  Light Microscopes 4<br \/>\n  Incubator Shaker 1<br \/>\n  Colorimeter 2<br \/>\n  Laminar Flow Chamber 2<br \/>\n  Glassware, Chemicals, Media as Required\n<\/p>\n<h4>Course Outcome:<\/h4>\n<p align=\"justify\">\n  At the end of the course the students will be able to,<\/p>\n<ol>\n<li>learn various qualitative and quantitative techniques<\/li>\n<li>describe various types of biochemical reaction<\/li>\n<li>evaluate the novelty of the experiment<\/li>\n<\/ol>\n<h4>Text Books:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Practical Biochemistry by R.C. Gupta and S. Bhargavan. I Edition 2014.<\/li>\n<li>Introduction of Practical Biochemistry by David T. Plummer. III Edition, 2017<\/li>\n<\/ol>\n<h4>References:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Harpers Biochemistry Ed. R.K. Murray, D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.24th edition 1996.<\/li>\n<li>Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers 6th edition 2015.<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Pharmaceutical Technology, 2019 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/pharma-tech+2nd-sem\">Pharma Tech 2nd Sem subject syllabuses for 2019 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Pharmaceutical Technology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University Pharma Tech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biochemistry Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Pharma Tech students. For [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[45,142],"tags":[],"class_list":["post-36746","post","type-post","status-publish","format-standard","hentry","category-2nd-sem","category-pharmaceutical-technology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/36746","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=36746"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/36746\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=36746"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=36746"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=36746"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}