{"id":35886,"date":"2021-05-25T06:03:22","date_gmt":"2021-05-25T06:03:22","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/ib5611-molecular-biology-and-genetic-engineering-lab-syllabus-for-industrial-biotech-6th-sem-2019-regulation-anna-university\/"},"modified":"2021-05-25T06:03:22","modified_gmt":"2021-05-25T06:03:22","slug":"ib5611-molecular-biology-and-genetic-engineering-lab-syllabus-for-industrial-biotech-6th-sem-2019-regulation-anna-university","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/ib5611-molecular-biology-and-genetic-engineering-lab-syllabus-for-industrial-biotech-6th-sem-2019-regulation-anna-university\/","title":{"rendered":"IB5611: Molecular Biology and Genetic Engineering Lab Syllabus for Industrial Biotech 6th Sem 2019 Regulation Anna University"},"content":{"rendered":"<p align=\"justify\">Molecular Biology and Genetic Engineering Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna Universities<\/a> official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Industrial Technology 6th Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/industrial-technology-industrial-biotech-syllabus-for-6th-sem-2019-regulation-anna-university\">Industrial Biotech 6th Sem 2019 regulation scheme<\/a>. The detailed syllabus of molecular biology and genetic engineering lab is as follows. <\/p>\n<p>  <title>Molecular Biology and Genetic Engineering Lab<\/title><\/p>\n<h4>Course Objective:<\/h4>\n<p align=\"justify\">\n  The course aims to<\/p>\n<ul>\n<li>make the students to learn the basic DNA isolation techniques.<\/li>\n<li>learn about the identification and characterisation of gene and protein<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Isolation of total DNA<\/li>\n<li>Isolation of Plasmid DNA<\/li>\n<li>Agarose gel electrophoresis<\/li>\n<li>Restriction enzyme digestion of DNA<\/li>\n<li>DNA ligation<\/li>\n<li>Competent cell preparation and Transformation<\/li>\n<li>Blue-White screening<\/li>\n<li>Induction and Analysis of Gene expression<\/li>\n<li>PCR<\/li>\n<li>SDS-PAGE<\/li>\n<li>Western blot<\/li>\n<\/ol>\n<h4>Equipment Required:<\/h4>\n<p align=\"justify\">\n  (for 60 students)<\/p>\n<ul>\n<li>Micropipettes (0.5-2.5 pl) &#8211; 30 Nos.<\/li>\n<li>Micropipettes (2-20 pl) &#8211; 30 Nos.<\/li>\n<li>Micropipettes (20-200 pl) &#8211; 30 Nos.<\/li>\n<li>Micropipettes (100-1000 pl) &#8211; 30 Nos.<\/li>\n<li>Refrigerated centrifuge &#8211; 2 Nos.<\/li>\n<li>Chemical fume hoods (for handling toxic solvents) &#8211; 2 Nos.<\/li>\n<li>Temperature controlled Incubator shaker &#8211; 2 Nos.<\/li>\n<li>Temperature controlled water bath &#8211; 2 Nos.<\/li>\n<li>Ice flake machine &#8211; 2 Nos.<\/li>\n<li>Agarose gel apparatus with power packs &#8211; 30 Nos.<\/li>\n<li>Laminar Air flow (3 or 4 ft length) &#8211; 2 Nos.<\/li>\n<li>PCR machine (96\/48 Wells) &#8211; 2 Nos.<\/li>\n<li>SDS-PAGE apparatus &#8211; 30 Nos.<\/li>\n<li>Western transfer apparatus (Semi dry) &#8211; 2 Nos.<\/li>\n<li>Western transfer apparatus (wet) &#8211; 30 Nos.<\/li>\n<li>Glasswares \/ Plasticwares\/Chemicals\/Media as required<\/li>\n<\/ul>\n<h4>Course Outcome:<\/h4>\n<p align=\"justify\">\n  At the end of the course the students will be able to<\/p>\n<ol>\n<li>experience basic techniques of DNA isolation and manipulation<\/li>\n<li>experience in selecting genetically transformed organisms for downstream analysis.<\/li>\n<li>experience basic techniques involved in analysis of gene expression at nucleic acids and proteins level<\/li>\n<\/ol>\n<h4>References:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Green MR and Sambrook J. Molecular Cloning: A Laboratory Manual. 4th Edition, CSHL press, 2012.<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/industrial-biotech+6th-sem\">Industrial Biotech 6th Sem subject syllabuses for 2019 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Industrial Technology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University Industrial Biotech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Molecular Biology and Genetic Engineering Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[49,139],"tags":[],"class_list":["post-35886","post","type-post","status-publish","format-standard","hentry","category-6th-sem","category-industrial-biotechnology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35886","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=35886"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35886\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=35886"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=35886"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=35886"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}