{"id":35881,"date":"2021-05-25T06:03:19","date_gmt":"2021-05-25T06:03:19","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/ib5511-immunology-lab-syllabus-for-industrial-biotech-5th-sem-2019-regulation-anna-university\/"},"modified":"2021-05-25T06:03:19","modified_gmt":"2021-05-25T06:03:19","slug":"ib5511-immunology-lab-syllabus-for-industrial-biotech-5th-sem-2019-regulation-anna-university","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/ib5511-immunology-lab-syllabus-for-industrial-biotech-5th-sem-2019-regulation-anna-university\/","title":{"rendered":"IB5511: Immunology Lab Syllabus for Industrial Biotech 5th Sem 2019 Regulation Anna University"},"content":{"rendered":"<p align=\"justify\">Immunology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the <a class=\"rank-math-link\" href=\"https:\/\/cac.annauniv.edu\/\" style=\"color: inherit\" target=\"_blank\" rel=\"noopener\">Anna Universities<\/a> official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information,  do visit full semester subjects post given below. <\/p>\n<p align=\"justify\">For Industrial Technology 5th Sem scheme and its subjects, do visit <a class=\"rank-math-link\" href=\"..\/industrial-technology-industrial-biotech-syllabus-for-5th-sem-2019-regulation-anna-university\">Industrial Biotech 5th Sem 2019 regulation scheme<\/a>. The detailed syllabus of immunology lab is as follows. <\/p>\n<p>  <title>Immunology lab<\/title><\/p>\n<h4>Course Objective:<\/h4>\n<p align=\"justify\">\n  The course aims to<\/p>\n<ul>\n<li>learn the isolation and staining of different blood cells<\/li>\n<li>learn the different immunological assays<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Selection of animals and introduction to animal handling (mice\/rat\/rabbit\/ chicken\/fish etc)<\/li>\n<li>Determination of various routes of immunization<\/li>\n<li>Methods of bleeding, serum separation and storage<\/li>\n<li>Preparation of antigens\/immunization schedule for raising antisera.<\/li>\n<li>Identification of leukocytes from blood smear by differential staining (Geimsa stain)<\/li>\n<li>Enumeration of RBCs\/leukocytes by Neubar chamber for cell culture studies.<\/li>\n<li>Separation of Peripheral Blood Mononuclear Cells (PBMC) by Ficoll -Hypaque<\/li>\n<li>Determination of agglutination reaction: Blood grouping antigens<\/li>\n<li>Determination of immunoprecipitaion reaction: immunodiffusion\/immunoelectrophoresis<\/li>\n<li>Evaluation of antibody titre by ELISA method<\/li>\n<li>Diagnostic determination of agglutination: Widal test<\/li>\n<li>Rapid diagnostic tests: Immunochromatographic test strips*Animal experiments will be conducted by trained faculties in accordance with UGC requirements and CPCSEA guidelines and no dissection of animals will be done<\/li>\n<\/ol>\n<h4>Course Outcome:<\/h4>\n<p align=\"justify\">\n  At the end of the course the students will be able to<\/p>\n<ol>\n<li>gain experience basic designing of immunological tests.<\/li>\n<li>gain experience in isolation of different cells<\/li>\n<li>gain experience in handling of animals and its immunization<\/li>\n<\/ol>\n<h4>List of Equipments for 30 Students<\/h4>\n<p align=\"justify\">\n<ul>\n<li>Elisa reader 1<\/li>\n<li>Microscopes 8<\/li>\n<li>Microwave oven 1<\/li>\n<li>Hot plate 4<\/li>\n<li>Vortex mixer 4<\/li>\n<li>Table top refrigerated Centrifuge 1<\/li>\n<li>Fluorescent microsope 1<\/li>\n<\/ul>\n<h4>References:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Roitt I, Male, Brostoff. Immunology, Mosby Publ., 2002.<\/li>\n<li>Kuby J, Immunology, WH Freeman and Co., 7th edition 2013<\/li>\n<li>Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill, 1998.<\/li>\n<li>Antibodies: A Laboratory Manual, Second edition Edward A. Greenfield, Dana-Farber, Cancer Institute,Cold Spring Harbour Laboratory 2014<\/li>\n<li>Current protocols in Immunology Volume I-III John E. Coligan (Editor), Ada M. Kruisbeek (Editor), David H. Margulies (Editor), Ethan M. Shevach (Editor), Warren Strober (Editor) 2004<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/industrial-biotech+5th-sem\">Industrial Biotech 5th Sem subject syllabuses for 2019 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Industrial Technology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University Industrial Biotech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Immunology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[48,139],"tags":[],"class_list":["post-35881","post","type-post","status-publish","format-standard","hentry","category-5th-sem","category-industrial-biotechnology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35881","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=35881"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35881\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=35881"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=35881"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=35881"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}