{"id":35866,"date":"2021-05-25T06:03:10","date_gmt":"2021-05-25T06:03:10","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/ib5311-cell-biology-lab-syllabus-for-industrial-biotech-3rd-sem-2019-regulation-anna-university\/"},"modified":"2021-05-25T06:03:10","modified_gmt":"2021-05-25T06:03:10","slug":"ib5311-cell-biology-lab-syllabus-for-industrial-biotech-3rd-sem-2019-regulation-anna-university","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/ib5311-cell-biology-lab-syllabus-for-industrial-biotech-3rd-sem-2019-regulation-anna-university\/","title":{"rendered":"IB5311: Cell Biology Lab Syllabus for Industrial Biotech 3rd Sem 2019 Regulation Anna University"},"content":{"rendered":"

Cell Biology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities<\/a> official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below. <\/p>\n

For Industrial Technology 3rd Sem scheme and its subjects, do visit Industrial Biotech 3rd Sem 2019 regulation scheme<\/a>. The detailed syllabus of cell biology lab is as follows. <\/p>\n

Cell biology lab<\/title><\/p>\n<h4>Course Objective:<\/h4>\n<p align=\"justify\">\n The course aims to<\/p>\n<ul>\n<li>make the students learn about the principles of microscopy and sterilisation techniques<\/li>\n<li>train the students about different cell staining and viability methods<\/li>\n<\/ul>\n<h4>List of Experiments:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Introduction to principles of sterile techniques and cell propagation<\/li>\n<li>Principles of microscopy , phase contrast and fluorescent microscopy<\/li>\n<li>Identification of given plant, animal and bacterial cells and their components by microscopy<\/li>\n<li>Staining methods\n<ol type=\"a\">\n<li>Haematoxylin and Eosin<\/li>\n<li>Leishman staining<\/li>\n<li>Giemsa staining<\/li>\n<\/ol>\n<\/li>\n<li>Cell\/tissue lysis and fractionation\n<ol type=\"a\">\n<li>Homogenization<\/li>\n<li>Osmosis<\/li>\n<li>Differential centrifugation<\/li>\n<\/ol>\n<\/li>\n<li>Cell viability studies\n<ol type=\"a\">\n<li>Tryphan blue dye exclusion<\/li>\n<li>Using tetrazolium salts<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<h4>Course Outcome:<\/h4>\n<p align=\"justify\">\n At the end of the course the students will be able to<\/p>\n<ol>\n<li>develop the ability to propagate cells under in vitro conditions as well as their storage.<\/li>\n<li>carryout differential staining in order to understand the internal components\/complexities of a cell.<\/li>\n<li>evaluate the integrity and lysis of cells in culture for downstream experiments<\/li>\n<\/ol>\n<h4>Equipment Required:<\/h4>\n<p align=\"justify\">\n<h4>(For 60 Students)<\/h4>\n<ul>\n<li>Micropipettes (0.5-2.5 pl) – 30 Nos.<\/li>\n<li>Micropipettes (2-20 pl) – 30 Nos.<\/li>\n<li>Micropipettes (20-200 pl) – 30 Nos.<\/li>\n<li>Micropipettes (100-1000 pl) – 30 Nos.<\/li>\n<li>Refrigerated centrifuge – 2 Nos.<\/li>\n<li>Temperature controlled Incubator shaker – 2 Nos.<\/li>\n<li>Temperature controlled water bath – 2 Nos.<\/li>\n<li>Ice flake machine – 2 Nos.<\/li>\n<li>Phase contrast microscope (Upright) – 8 Nos.<\/li>\n<li>Phase contrast microscope (Inverted) – 2 Nos.<\/li>\n<li>Tissue homogenizer – 2 Nos<\/li>\n<li>Microplate reader – 1 No.<\/li>\n<li>Glass wares\/Plastic wares\/Chemicals\/Media as required<\/li>\n<\/ul>\n<h4>Text Books:<\/h4>\n<p align=\"justify\">\n<ol>\n<li>Rickwood, D. and J.R. Harris Cell Biology : Essential Techniques, John Wiley, 1996.<\/li>\n<li>Davis, J.M. Basic Cell Culture : A Practical Approach, IRL, 1994.<\/li>\n<\/ol>\n<p align=\"justify\">For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit <a class=\"rank-math-link\" href=\"..\/category\/industrial-biotech+3rd-sem\">Industrial Biotech 3rd Sem subject syllabuses for 2019 regulation<\/a>. <\/p>\n<p align=\"justify\">For all Industrial Technology results, visit <a class=\"rank-math-link\" href=\"https:\/\/www.inspirenignite.com\/anna-university\/anna-university-results\/\">Anna University Industrial Biotech all semester results<\/a> direct link. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cell Biology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. […]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[46,139],"tags":[],"class_list":["post-35866","post","type-post","status-publish","format-standard","hentry","category-3rd-sem","category-industrial-biotechnology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=35866"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/35866\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=35866"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=35866"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=35866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}