Genetic Engineering and Genomics for Biotechnologists Syllabus for B.Tech Sixth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.<\/p>\n
The detailed syllabus for Total Quality Management for Genetic Engineering and Genomics B.Tech 2016-2017 (R13) sixthsem is as follows<\/p>\n
OBJECTIVES:<\/strong><\/p>\n UNIT I : BASICS OF RECOMBINANT DNA TECHNOLOGY<\/strong> \u00a0 \u00a0 \u00a0[9 Hours] UNIT II : DNA LIBRARIES \u00a0<\/strong> \u00a0 \u00a0\u00a0[9 Hours] UNIT III : SEQUENCING AND AMPLIFICATION OF DNA<\/strong> \u00a0 \u00a0\u00a0[9 Hours]\n
\nproduction of recombinant proteins and PCR techniques.<\/li>\n
\nManipulation of DNA \u2013 Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for yeast, insect and mammalian systems, Prokaryotic and eukaryotic expression host systems, Introduction of recombinant DNA in to host cells and selection methods.<\/p>\n
\nConstruction of genomic and cDNA libraries, Artificial chromosomes \u2013 BACs and
\nYACs, Chromosome walking, Screening of DNA libraries using nucleic acid probes and antisera.<\/p>\n
\nMaxam Gilbert\u2019s and Sanger Coulson\u2019s and automated methods of DNA sequencing, Inverse PCR, Nested PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Hot start PCR, Colony PCR, single cell PCR, Real-time PCR\/qPCR \u2013 SYBR green assay, Taqman assay, Molecular beacons, Site directed mutagenesis.<\/p>\n