{"id":21752,"date":"2019-12-10T07:46:20","date_gmt":"2019-12-10T07:46:20","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/downstream-processing-laboratory-biotech-7th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/"},"modified":"2019-12-10T07:46:20","modified_gmt":"2019-12-10T07:46:20","slug":"downstream-processing-laboratory-biotech-7th-sem-syllabus-for-b-tech-2017-regulation-anna-univ","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/downstream-processing-laboratory-biotech-7th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/","title":{"rendered":"Downstream Processing Laboratory Biotech 7th Sem Syllabus for B.Tech 2017 Regulation Anna Univ"},"content":{"rendered":"<p>Downstream Processing Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from <a href=\"https:\/\/www.annauniv.edu\/\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for students of Anna University. The details of the course are: course code (BT8711), Category (PC), Contact Periods\/week (4), Teaching hours\/week (0), Practical Hours\/week (0). The total course credits are 4.<\/p>\n<p>For all other biotech 7th sem syllabus for b.tech 2017 regulation anna univ you can visit <a href=\"..\/biotech-7th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\">Biotech 7th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects<\/a>. The detail syllabus for downstream processing laboratory is as follows.&#8221;<\/p>\n<p><h4>Course Objective:<\/h4>\n<ul>\n<li>To provide hands on training in Down stream processing through simple experimentations in the laboratory. This will be a pre-requisite for project work.<\/li>\n<p>The objectives of this course is to practice the students<\/p>\n<li>To understand the nature of the end product, its concentration, stability and degree of purification required<\/li>\n<li>To design processes for the recovery and subsequent purification of target biological products.<\/li>\n<\/ul>\n<p><h4>List of Experiments:<\/h4>\n<ol>\n<li>Solid liquid separation &#8211; centrifugation<\/li>\n<li>Solid liquid separation &#8211; microfiltration<\/li>\n<li>Cell disruption techniques &#8211; ultrasonication or French pressure cell or Dynomill<\/li>\n<li>Precipitation &#8211; ammonium sulphite precipitation<\/li>\n<li>Ultra filtration separation<\/li>\n<li>Aqueous two phase extraction of biologicals<\/li>\n<li>High resolution purification &#8211; affinity chromatography<\/li>\n<li>High resolution purification &#8211; ion exchange chromatography<\/li>\n<li>Product polishing &#8211; spray drying or freeze drying<\/li>\n<li>Size exclusion chromatography<\/li>\n<\/ol>\n<p><h4>List of Equipment for 30 Students<\/h4>\n<ul>\n<li>Centrifuge\t1<\/li>\n<li>Cross flow filtration set up\t2<\/li>\n<li>FPLC\t1<\/li>\n<li>Sonicator or French press or Dynomill\t1<\/li>\n<\/ul>\n<p><h4>Course Outcome:<\/h4>\n<p>Upon success completion of this course, the students would have<\/p>\n<ul>\n<li>Acquired knowledge for the separation of whole cells and other insoluble ingredients from the culture broth.<\/li>\n<li>Learned cell disruption techniques to release intracellular products<\/li>\n<li>Learned various techniques like evaporation, extraction, precipitation, membrane separation for concentrating biological products<\/li>\n<li>Learned the basic principles and techniques of chromatography to purify the biological products and formulate the products for different end uses.<\/li>\n<\/ul>\n<p><h4>References:<\/h4>\n<ol>\n<li>P.A. Belter, E.L. Cussler And Wei-Houhu &#8211; Bioseparations &#8211; Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).<\/li>\n<li>R.O. Jenkins, (Ed.) &#8211; Product Recovery In Bioprocess Technology &#8211; Biotechnology ByOpen Learning Series, Butterworth-Heinemann (1992).<\/li>\n<li>J.C. Janson And L. Ryden, (Ed.) &#8211; Protein Purification &#8211; Principles, High ResolutionMethods And Applications, VCH Pub. 1989.<\/li>\n<\/li>\n<\/ol>\n<p>For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit <a href=\"..\/category\/biotech+7th-sem\">Biotech 7th Sem syllabus for 2017 Regulation<\/a>.<\/p>\n<p>Dont forget to <a href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" target=\"_blank\" rel=\"noopener\">download iStudy<\/a> for latest syllabus and results, class timetable and more.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Downstream Processing Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[50,81],"tags":[],"class_list":["post-21752","post","type-post","status-publish","format-standard","hentry","category-7th-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21752","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=21752"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21752\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=21752"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=21752"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=21752"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}