{"id":21732,"date":"2019-12-10T07:46:08","date_gmt":"2019-12-10T07:46:08","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/genetic-engineering-laboratory-biotech-6th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/"},"modified":"2019-12-10T07:46:08","modified_gmt":"2019-12-10T07:46:08","slug":"genetic-engineering-laboratory-biotech-6th-sem-syllabus-for-b-tech-2017-regulation-anna-univ","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/genetic-engineering-laboratory-biotech-6th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/","title":{"rendered":"Genetic Engineering Laboratory Biotech 6th Sem Syllabus for B.Tech 2017 Regulation Anna Univ"},"content":{"rendered":"<p>Genetic Engineering Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from <a href=\"https:\/\/www.annauniv.edu\/\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for students of Anna University. The details of the course are: course code (BT8612), Category (PC), Contact Periods\/week (4), Teaching hours\/week (0), Practical Hours\/week (0). The total course credits are 4.<\/p>\n<p>For all other biotech 6th sem syllabus for b.tech 2017 regulation anna univ you can visit <a href=\"..\/biotech-6th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\">Biotech 6th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects<\/a>. The detail syllabus for genetic engineering laboratory is as follows.&#8221;<\/p>\n<p><h4>Course Objective:<\/h4>\n<ul>\n<li>Provide hands-on experience in performing basic recombinant DNA techniques.<\/li>\n<li>Introduce students to the theory behind in each techniques and to describe common applications of each methodology in biological research.<\/li>\n<\/ul>\n<p><h4>List of Experiments:<\/h4>\n<ol>\n<li>Preparation of plasmid DNA<\/li>\n<li>Elution of DNA from agarose gels<\/li>\n<li>Restriction digestion<\/li>\n<li>Ligation of DNA into expression vectors<\/li>\n<li>Transformation&amp; Selection of recombinants &#8211; Blue white screening assay<\/li>\n<li>Optimisation of time of inducer for recombinant protein expression<\/li>\n<li>Expression of protein profiling by SDS &#8211; PAGE<\/li>\n<li>Western blotting, Southern blotting<\/li>\n<li>PCR amplification of genes<\/li>\n<li>Colony lysate PCR.<\/li>\n<\/ol>\n<p><h4>Course Outcome:<\/h4>\n<p>By the end of this course, students should be able to:<\/p>\n<ul>\n<li>Describe the main principles, methods for preparation and cloning of DNA in various organisms.<\/li>\n<li>Express clearly about the gene amplification and methods for analysis of DNA, such as hybridization, restriction analysis and gene expressions.<\/li>\n<li>Use genetic and biotechnological techniques to manipulate genetic materials and develops new and improved living organisms.<\/li>\n<li>Students will be aware of the hazardous chemicals and safety precautions in case of emergency.<\/li>\n<\/ul>\n<p><h4>Equipment Needed for 30 Students<\/h4>\n<ul>\n<li>Electrphoresis Kit\t1<\/li>\n<li>PCR\t1<\/li>\n<li>Incubators\t2<\/li>\n<li>Light Microscopes\t4<\/li>\n<li>Incubator Shaker\t1<\/li>\n<li>Spectrophotometer\t2<\/li>\n<li>Laminar Flow Chamber\t2<\/li>\n<li>Glassware, Chemicals, Media as required<\/li>\n<\/ul>\n<p><h4>References:<\/h4>\n<ol>\n<li>Old RW, Primrose SB, Principles Of Gene Manipulation, An Introduction To Genetic Engineering , Blackwell Science Publications, 1993.<\/li>\n<li>Ansubel FM, Brent R, Kingston RE, Moore DD, Current Protocols In Molecular Biology , Greene Publishing Associates, NY, 1988.<\/li>\n<li>Berger Sl, Kimmer AR, Methods In Enzymology, Vol 152, Academic Press, 1987<\/li>\n<\/li>\n<\/ol>\n<p>For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit <a href=\"..\/category\/biotech+6th-sem\">Biotech 6th Sem syllabus for 2017 Regulation<\/a>.<\/p>\n<p>Dont forget to <a href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" target=\"_blank\" rel=\"noopener\">download iStudy<\/a> for latest syllabus and results, class timetable and more.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Genetic Engineering Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[49,81],"tags":[],"class_list":["post-21732","post","type-post","status-publish","format-standard","hentry","category-6th-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21732","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=21732"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21732\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=21732"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=21732"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=21732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}