{"id":21670,"date":"2019-12-10T07:45:32","date_gmt":"2019-12-10T07:45:32","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/biochemistry-laboratory-biotech-2nd-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/"},"modified":"2019-12-10T07:45:32","modified_gmt":"2019-12-10T07:45:32","slug":"biochemistry-laboratory-biotech-2nd-sem-syllabus-for-b-tech-2017-regulation-anna-univ","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/biochemistry-laboratory-biotech-2nd-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/","title":{"rendered":"Biochemistry Laboratory Biotech 2nd Sem Syllabus for B.Tech 2017 Regulation Anna Univ"},"content":{"rendered":"<p>Biochemistry Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from <a href=\"https:\/\/www.annauniv.edu\/\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for students of Anna University. The details of the course are: course code (BT8261), Category (PC), Contact Periods\/week (4), Teaching hours\/week (0), Practical Hours\/week (0). The total course credits are 4.<\/p>\n<p>For all other biotech 2nd sem syllabus for b.tech 2017 regulation anna univ you can visit <a href=\"..\/biotech-2nd-sem-syllabus-for-b-tech-2017-regulation-anna-univ\">Biotech 2nd Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects<\/a>. The detail syllabus for biochemistry laboratory is as follows.&#8221;<\/p>\n<p><h4>Aims:<\/h4>\n<ul>\n<li>To learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of the same in the body fluids.<\/li>\n<\/ul>\n<p><h4>List of Experiments:<\/h4>\n<ol>\n<li>General guidelines for working in biochemistry lab (theory)<\/li>\n<li>Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.<\/li>\n<li>Accuracy, precision, sensitivity and specificity (theory)<\/li>\n<li>Preparation of buffer -titration of a weak acid and a weak base.<\/li>\n<li>Qualitative tests for carbohydrates &#8211; distinguishing reducing from non-reducing sugars and keto from aldo sugars.<\/li>\n<li>Quantitative method for amino acid estimation using ninhydrin &#8211; distinguishing amino from imino acid.<\/li>\n<li>Protein estimation by Biuret and Lowrys methods.<\/li>\n<li>Protein estimation by Bradford and spectroscopic methods.<\/li>\n<li>Extraction of lipids and analysis by TLC.<\/li>\n<li>Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).<\/li>\n<li>Enzymatic assay: phosphatase from potato.<\/li>\n<li>Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.<\/li>\n<\/ol>\n<p><h4>Equipment Needed for 20 Students<\/h4>\n<ul>\n<li>Autocalve\t1<\/li>\n<li>Hot Air Oven\t1<\/li>\n<li>Incubators\t2<\/li>\n<li>Light Microscopes\t4<\/li>\n<li>Incubator Shaker\t1<\/li>\n<li>Colorimeter\t2<\/li>\n<li>Laminar Flow Chamber\t2<\/li>\n<li>Glassware, Chemicals, Media as required<\/li>\n<\/ul>\n<p><h4>Text Books:<\/h4>\n<ol>\n<li>Practical Biochemistry by R.C. Gupta and S. Bhargavan.<\/li>\n<li>Introduction of Practical Biochemistry by David T. Phummer. (II Edition)<\/li>\n<\/ol>\n<p><h4>References:<\/h4>\n<ol>\n<li>Harpers Biochemistry Ed. R.K. Murray , D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.<\/li>\n<li>Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers<\/li>\n<\/li>\n<\/ol>\n<p>For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit <a href=\"..\/category\/biotech+2nd-sem\">Biotech 2nd Sem syllabus for 2017 Regulation<\/a>.<\/p>\n<p>Dont forget to <a href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" target=\"_blank\" rel=\"noopener\">download iStudy<\/a> for latest syllabus and results, class timetable and more.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biochemistry Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[45,81],"tags":[],"class_list":["post-21670","post","type-post","status-publish","format-standard","hentry","category-2nd-sem","category-biotech"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21670","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=21670"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21670\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=21670"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=21670"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=21670"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}