{"id":21069,"date":"2019-12-10T07:21:41","date_gmt":"2019-12-10T07:21:41","guid":{"rendered":"https:\/\/www.inspirenignite.com\/anna-university\/molecular-biology-and-genetic-engineering-laboratory-pharma-4th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/"},"modified":"2019-12-10T07:21:41","modified_gmt":"2019-12-10T07:21:41","slug":"molecular-biology-and-genetic-engineering-laboratory-pharma-4th-sem-syllabus-for-b-tech-2017-regulation-anna-univ","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/anna-university\/molecular-biology-and-genetic-engineering-laboratory-pharma-4th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\/","title":{"rendered":"Molecular Biology and Genetic Engineering Laboratory Pharma 4th Sem Syllabus for B.Tech 2017 Regulation Anna Univ"},"content":{"rendered":"<p>Molecular Biology and Genetic Engineering Laboratory detail syllabus for Pharmacy (Pharma), 2017 regulation is taken from <a href=\"https:\/\/www.annauniv.edu\/\" target=\"_blank\" rel=\"noopener\">Anna University<\/a> official website and presented for students of Anna University. The details of the course are: course code (PY8412), Category (PC), Contact Periods\/week (4), Teaching hours\/week (0), Practical Hours\/week (0). The total course credits are 4.<\/p>\n<p>For all other pharma 4th sem syllabus for b.tech 2017 regulation anna univ you can visit <a href=\"..\/pharma-4th-sem-syllabus-for-b-tech-2017-regulation-anna-univ\">Pharma 4th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects<\/a>. The detail syllabus for molecular biology and genetic engineering laboratory is as follows.&#8221;<\/p>\n<p><h4>Course Objective:<\/h4>\n<ul>\n<li>To understand the principle of nucleic acid isolation.<\/li>\n<li>To understand the principles of PCR and their uses in genetic engineering.<\/li>\n<li>To gain a thorough knowledge about nucleic acid hybridization.<\/li>\n<li>To learn history of DNA sequencing and current methods and gene synthesis<\/li>\n<\/ul>\n<p><h4>List of Experiments:<\/h4>\n<ol>\n<li>Preparation of Genomic DNA<\/li>\n<li>PCR amplification of gene from the genomic DNA<\/li>\n<li>Preparation of plasmid DNA<\/li>\n<li>Detection of Plasmid DNA by Agarose gel electrophoresis<\/li>\n<li>Restriction Digestion of the vector and Insert<\/li>\n<li>Ligation and Transformation ofE.coli<\/li>\n<li>Lysate PCR confirmation.<\/li>\n<li>Restriction and gel elution of DNA fragments<\/li>\n<li>Electroporation of Yeast<\/li>\n<li>SDS-PAGE analysis of purified protein<\/li>\n<li>Western blot confirmation of expressed protein (anti his)<\/li>\n<li>ELISA- Quantification of proteins.<\/li>\n<li>RNA Isolation<\/li>\n<li>cDNA preparation from RNA<\/li>\n<li>Site directed mutagenesis<\/li>\n<li>Southern hybridization experiment<\/li>\n<ul>\n<li>PCR machine<\/li>\n<li>Electrophoretic assemblies for DNA and protein separations<\/li>\n<li>ELISA reader<\/li>\n<li>Ultracentrifuge<\/li>\n<li>Laminar air flow cabinets<\/li>\n<li>Cooling centrifuge<\/li>\n<\/ul>\n<\/ol>\n<p><h4>Course Outcome:<\/h4>\n<p>By the end of this course, students will be able to:<\/p>\n<ul>\n<li>Describe the main principles, methods for preparation and cloning of DNA in various organisms.<\/li>\n<li>Express clearly about the gene amplification and methods for analysis of DNA, such as hybridization, restriction analysis and gene expressions.<\/li>\n<li>Express clearly about the analysis of protein expressions.<\/li>\n<\/ul>\n<p><h4>References:<\/h4>\n<ol>\n<li>Old RW, Primrose SB, Principles of Gene Manipulation, An Introduction to GeneticEngineering , Blackwell Science Publications, 1993.<\/li>\n<li>Ansubel FM, Brent R, Kingston RE, Moore DD, Current Protocols in Molecular Biology, Greene Publishing Associates, NY, 2003<\/li>\n<li>S. John Vennison, Laboratory manual for Genetic Engineering, Eastern economy edition, 2009.<\/li>\n<\/li>\n<\/ol>\n<p>For detail syllabus of all other subjects of B.Tech Pharma, 2017 regulation do visit <a href=\"..\/category\/pharma+4th-sem\">Pharma 4th Sem syllabus for 2017 Regulation<\/a>.<\/p>\n<p>Dont forget to <a href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" target=\"_blank\" rel=\"noopener\">download iStudy<\/a> for latest syllabus and results, class timetable and more.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Molecular Biology and Genetic Engineering Laboratory detail syllabus for Pharmacy (Pharma), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of [&hellip;]<\/p>\n","protected":false},"author":2297,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[47,90],"tags":[],"class_list":["post-21069","post","type-post","status-publish","format-standard","hentry","category-4th-sem","category-pharma"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21069","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/users\/2297"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/comments?post=21069"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/posts\/21069\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/media?parent=21069"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/categories?post=21069"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/anna-university\/wp-json\/wp\/v2\/tags?post=21069"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}